PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex
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چکیده
منابع مشابه
New PCR Assays for the Identification of Fusarium verticillioides, Fusarium subglutinans, and Other Species of the Gibberella fujikuroi Complex
Fusarium verticillioides and Fusarium subglutinans are important fungal pathogens of maize and other cereals worldwide. In this study, we developed PCR-based protocols for the identification of these pathogens targeting the gaoB gene, which codes for galactose oxidase. The designed primers recognized isolates of F. verticillioides and F. subglutinans that were obtained from maize seeds from sev...
متن کاملMorphological and phylogenetic investigation on selected Fusarium species belong to Gibberella fujikuroi species complex in Iran
The Gibberella fujikuroi species complex with Fusarium anamorphs is the causal of diverse diseases of many important plants. Many of the known species in this species complex produce a wide range of secondary metabolites. In this study, morphological characteristics of 46 isolates from rice, maize, sugarcane and onion and phylogenetic relationships of 22 isolates on the basis of ITS1-5.8S-ITS2 ...
متن کاملIsozyme Variation among Biological Species in the Gibberella fujikuroi Species Complex (Fusarium Section Liseola).
Isozyme phenotypes were determined for 101 strains of Gibberella fujikuroi and 2 strains of Gibberella nygamai that represent seven biological species (mating populations) isolated from a variety of plant hosts in dispersed geographic locations. Fourteen enzymes were resolved in one or more of three buffer systems. Two of the enzymes, arylesterase and acid phosphatase, were polymorphic within t...
متن کاملMolecular Identification of Fusarium Species in Gibberella fujikuroi Species Complex from Rice, Sugarcane and Maize from Peninsular Malaysia
The objective of this study was to identify Fusarium species in the Gibberella fujikuroi species complex from rice, sugarcane and maize as most of the Fusarium species in the species complex are found on the three crops. Isolates used were collected from the field and obtained from culture collection. The Fusarium isolates were initially sorted based on morphology and identifications confirmed ...
متن کاملRegulation of Gibberellin Biosynthesis in Gibberella fujikuroi.
Gibberellin production by Gibberella fujikuroi started only after the nitrogen source was depleted and ceased upon its renewal. Nitrogen repression of gibberellin biosynthesis is not an indirect effect of the growth arrest that follows the depletion of an essential nutrient because gibberellins were not produced upon depletion of phosphate. Mycelia produced gibberellins when suspended in a gluc...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2000
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.66.10.4378-4382.2000